compounds l2130 anti cancer metabolism compound library targetmol  (TargetMol)

Journal: Journal of Translational Medicine

Article Title: Deficiency of SDHC promotes metastasis by reprogramming fatty acid metabolism in colorectal cancer

doi: 10.1186/s12967-024-05361-x

Figure Lengend Snippet: Bioinformatics analysis. a The volcano plot represents the DEGs. b GO annotations of the DEGs. The Bulb map presents enrichment scores of the top 10 significantly enriched GO terms in biological processes. c GO annotations of the DEGs. The Bulb map presents enrichment scores of the top 10 significantly enriched GO terms in cellular components. d GO annotations of the DEGs. The Bulb map presents the enrichment scores of the top 10 significantly enriched GO terms in molecular functions. e KEGG pathway enrichment analysis of DEGs. f Proteasome pathway enriched by GSEA. g Parkinson disease pathway enriched by GSEA. h Fatty acid metabolism pathway enriched by GSEA. i Oxidative phosphorylation pathway enriched by GSEA. j butanoate metabolism pathway enriched by GSEA. k Citrate cycle (TCA cycle) pathway enriched by GSEA. Data are presented as the mean ± SD and analyzed by Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001

Article Snippet: Anti-cancer Metabolism Compound Library containing 237 anti-cancer metabolism compounds was purchased from TargetMol(L2130).

Techniques:

Journal: Journal of Translational Medicine

Article Title: Deficiency of SDHC promotes metastasis by reprogramming fatty acid metabolism in colorectal cancer

doi: 10.1186/s12967-024-05361-x

Figure Lengend Snippet: SDHC reprogramed fatty acid metabolism by regulating the PI3K/AKT pathways. a Western blotting was used to demonstrate the effects of silencing SDHC on the expression of critical enzymes involved in lipid metabolism, namely ACOX1, CPT1A, and ALDH3A2. b Western blotting was used to demonstrate the effects of overexpression SDHC on the expression of critical enzymes involved in lipid metabolism. c Migration assay analysis of orlistat in HCT116 and SW480 cells with SDHC decreased. d The effect of orlistat on liver metastasis formation in nude mice bearing HCT116 cells with SDHC decreased. e Western blotting reveals the levels of PI3K, p-PI3K, AKT and p-AKT in HCT116 and SW480 cells with SDHC decreased and control. f Western blotting demonstrates the levels of PI3K, p-PI3K, AKT and p-AKT in HCT116 and SW480 cells with SDHC overexpress and control. g Western blotting demonstrates the levels of AKT, p-AKT, ACOX1, CPT1A and ALDH3A2 in HCT116-sh-SDHC and SW480-sh-SDHC cells treated with MK-2206. h Migration assay analysis of MK-2206 in HCT116 and SW480 cells with SDHC decreased. i Schematic depiction of the regulation on fatty acid metabolism by SDHC in CRC cells. Data are presented as the mean ± SD and analyzed by Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001

Article Snippet: Anti-cancer Metabolism Compound Library containing 237 anti-cancer metabolism compounds was purchased from TargetMol(L2130).

Techniques: Western Blot, Expressing, Over Expression, Migration